DHA canola publications

The following are the key publications for CSIRO’s work on the development of DHA canola.

Patents

Petrie J, et al., 2008, Enzymes and methods for producing omega-3 fatty acids. PCT.

Miller M, et al., 2007, Feedstuffs for aquaculture comprising stearidonic acid feedstuffs for aquaculture. WO2007056823.

Singh S, et al., 2005. Synthesis of Long-Chain Polyunsaturated Fatty Acids by Recombinant Cells. WO2005103253.

Papers

Petrie J, et al. 2010, Isolation of three novel LC-PUFA delta-9 elongases and the transgenic assembly of the entire Pavlova salina DHA pathway in Nicotiana benthamiana. J Phycology In Press.

Petrie, J.R. et al. 2010, Metabolic engineering of omega-3 long-chain polyunsaturated fatty acids in plants using an acyl-CoA Delta6-desaturase with omega3-preference from the marine microalga Micromonas pusilla. Metab. Eng 12: 233-240.

Petrie J, et al. 2010, Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production. Plant Methods, 6: 8.

Nichols PD, et al. 2010, Long-Chain Omega-3 Oils – an update on sustainable sources, Nutrients 2: 572-585.

Wood CC, et al. 2009, A leaf-based assay using interchangeable design principles to rapidly assemble multistep recombinant pathways. Plant Biotechnol. J, 7: 914-924.

Naylor RL, et al. 2009, Feeding aquaculture in an era of finite resources. Proc Natl Acad Sci USA, 106: 15103-15110.

Drazen JC, Phleger CF, Guest MA, Nichols PD, 2009, Lipid composition and diet inferences in abyssal macrourids of the eastern North Pacific. Mar Ecol Prog Ser, 387: 1-14.

Chandrapavan A, Guest MA, Nichols PD, Gardner C, 2009, Translocating southern rock lobsters (Jasus edwardsii) from deep-water to shallow inshore water enhances nutritional condition through omega-3 long-chain polyunsaturated fatty acid content. J Expl Marine Biol & Ecol, 375: 9-15.

Robert S. et al. 2009, Isolation and characterisation of a Δ5-fatty acid elongase from the marine microalga Pavlova salina. Marine Biotechnology 11: 410-418.

Kitessa SM, Young P, 2009, Echium oil is better than rapeseed oil in enriching poultry meat with n-3 polyunsaturated fatty acids, including eicosapentaenoic acid and docosapentaenoic acid. Br. J. Nutr, 101: 709-715.

Petrie, J. et al. 2009, Isolation and characterisation of a high-efficiency desaturase and elongases from microalgae for transgenic LC-PUFA production. Marine Biotechnology, doi:10.1007/s10126-009-9230-1

Ho Ling K, Nichols PD, But PP, 2009, Fish-induced keriorrhea, Adv Food Nutr Res, 57: 1-52.

Drazen JC, Phleger CF, Guest MA, Nichols PD, 2008, Lipid, sterols and fatty acids of abyssal polychaetes, crustaceans, and a cnidarian from the northeast Pacific Ocean: food web implications. Mar Ecol Prog Ser, 372: 157-167.

Miller MR, Nichols PD, Carter CG, 2008, n-3 Oil sources for use in aquaculture – alternatives to the unsustainable harvest of wild fish. Nutr Res Rev, 21: 85-96.

Miller MR, Bridle AR, Nichols PD, Carter CG, 2008, Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance longchain (n-3) content of seawater Atlantic salmon (Salmo salar L.). J. Nutr, 138: 2179-2185.

Drazen JC, Phleger CF, Guest MA, Nichols PD, 2008, Lipid, sterols and fatty acid composition of abyssal holothurians and ophiuroids from the North-East Pacific Ocean: food web implications. Comp. Biochem. Physiol. B, Biochem. Mol. Biol 151: 79-87.

Nichols PD, Mancuso Nichols CA, 2008, Microbial signature lipid profiling and exopolysaccharides: experiences initiated with Professor David C White and transported to Tasmania, Australia. J. Microbiol. Methods 74: 33-46.

Miller MR, Nichols PD, Carter CG, 2008, The digestibility and accumulation of dietary phytosterols in Atlantic salmon (Salmo salar L.) smolt fed diets with replacement plant oils. Lipids 43: 549-557..

Miller MR, Nichols PD, Carter CG, 2007, Replacement of fish oil with thraustochytrid Schizochytrium sp. L oil in Atlantic salmon parr (Salmo salar L) diets. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol 148: 382-392.

Zhou X, et al. 2007, Isolation and characterization of genes from the marine microalga Pavlova salina encoding three front-end desaturases involved in docosahexaenoic acid biosynthesis. Phytochem. 68: 785-796.

Miller MR, Nichols PD, Carter CG, 2007, Replacement of dietary fish oil for Atlantic salmon parr (Salmo salar L.) with a stearidonic acid containing oil has no effect on omega-3 long-chain polyunsaturated fatty acid concentrations. Comp. Biochem. Physiol. B, Biochem. Mol. Biol 146: 197-206.

Miller MR, et al. 2006, Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of Atlantic salmon (Salmo salar L.) grown at elevated temperature. Lipids 41: 865-876.

Robert S, et al. 2005, Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil. Funct Plant Biol. 32: 473-479.